Daya Hambat Ekstrak Apel Manalagi (Malus sylvestris Mill.) terhadap Pertumbuhan Porphyromonas gingivalis

Abstract

Background: Periodontal disease is an inflammation of supporting tooth tissue and if left untreated, tooth loss might occur. One of bacteria that cause periodontal disease is P. gingivalis. The infection can be treated by synthetic or natural antibacterial. One of natural antibacterial ingredients that can subtitute synthetic antibacterial is Manalagi apple. Objective: To determine the inhibitory effect of Manalagi apple extract on the growth of P. gingivalis and Manalagi apple extract smallest concentration which still be able to inhibit the growth of P. gingivalis. Methods: The study was in vitro laboratory experiment with the pre-post test only control group design. The inhibition test used paper disc diffusion method. The inhibition effect was shown by the inhibition zone that as clear region at surrounding the paper disc. The sample was into 5 groups: positive control, negative control, and Manalagi apple extract consisted of 100%, 50% and 25% concentration. Results: The results showed that the Manalagi apple extract concentration of 100% and 50% had inhibition zone, but concentration of 25% had no inhibition zone. Conclusion: Manalagi apple extract was able to inhibit the growth of P. gingivalis bacteria and the minimum concentration of Manalagi apple extract which can inhibit the growth of P. gingivalis was the concentration of 50%.