Rapid Propagation of Virus-free Sugarcane (Saccharum officinarum) by Somatic Embryogenesis

Abstract

Elimination Sugarcane Mozaic Virus (SCMV) in sugarcane plantlets and propagation via somatic embryogenesis was assessed. The aim of this study was to obtain a virus-free sugarcane and determine methods for propagation via somatic embryogenesis. Explants derived from sugarcane SUT-02 plantlets. The explants were cultured on ribavirin and acyclovir medium at 0, 20 and 40 mgl -1 2,3) concentrations. Virus-free plantlets detected by DAS-ELISA. Rapid propagation virus-free plantlets through somatic embryogenesis consists of 3 stages. Stage 1: callus induction on the media (a) MSo, (b) MSo + 3 mgl -1

2,4-D, (c) MSo + 4 mgl

2,4-D, (d) MSo + 3 mgl -1 2,4-D + 1.5 mgl -1 BAP, (e) MSo + 4 mgl -1 2,4-D + 1.5 mgl -1 BAP, stage 2: proliferation on media MSo + 1.5 mgl -1 2,4-D and stage 3: regeneration on MSo media. The results showed that the media ribavirin and acyclovir at 20 mgl

and 40 mgl -1 concentration produces 100% virus-free sugarcane plantlets. Best embryogenic callus induction media is MSo + 3 mgl

-1 2,4-D and virus free plantlets have produced as much as ± 35 plantlets within 9 weeks.