Isolation And Hydrolysis Xylan From Soybean Waste With Endo-β1,4-D-Xilanase Of Bacillus sp. From Soil Termite Abdomen

Abstract

Soybean waste is by product in production of tofu and soymilk. Soybean waste commonly dumps directly to the water sewer and made environmental problems like eutrotification. Soybean waste contains 23% hemicellulose, 16% cellulose dan 28% protein. Xylan rich hemicellulose is potential source of xylooligosacharides (XOS). XOS was known as functional food with prebiotic activities. In this research, xylan was isolated from soybean waste then hydrolyzed by endoxylanase enzyme of Bacillus sp from soil termite abdominal. At the beginning, Soybean waste was processed by reflux to exclude lipid compound. Then process continued by alkaline treatment using NaOH 4-18% to separate the xylan from lignocellulose complex. Isolated xylan was hydrolyzed by endo-β-1,4-D-xylanase to get XOS. Hydrolised product analysed by TLC and HPLC. TLC analysis shown thick spot confirmed as xylopentaose (X5) compare to the Rf of the standard compound. HPLC data supported these analyses with the result of X5 as highest component (6959,88 ppm) beside the other XOS such as xylobiosa (X2) 6,34 ppm and xylosa (X1) 7,1 ppm. With the same enzyme, the result of hydrolyzed of soybean wastes xylan is not different with hydrolysed of oat spelt xylan, the common xylan source.