DETERMINATION OF TOTAL PHENOLIC CONTENT AND ANTIOXIDANT ACTIVITY IN METHANOLIC EXTRACT OF ROBUSTA AND ARABICA COFFEE LEAVES

Abstract

Phenols contained in coffee beans may also found in the coffee leaves. Previous research showed that the arabica coffee leaves contain phenolic compounds [1]. Phenolic compounds are compounds that have one or more hydroxyl groups attached directly to an aromatic ring [2]. Phenolics compounds have many biological effects, including antioxidant activity [3]. Antioxidants are the first line for defense against free radical damage, and important for health. The need for antioxidants becomes even more critical with increased exposure to free radicals. Pollution, cigarette smoke, drugs, illness, stress, and even exercise can increase free radical exposure [4]. The aim of this study was to determine total phenolic content and antioxidant activity in methanolic extracts of robusta and arabica coffee leaves (old and young leaves), as potential sources of natural antioxidants. The relationship between phenolic content and antioxidant activity was also investigated.