Arcangelisia flava LEAVES ETHANOLIC EXTRACT SUPPRESSES CANCER CELL LINES VIA NON APOPTOTIC PATHWAY

Abstract

Arcangelisia flava is a potential candidate to be developed as cancer chemoprevention agent. A. flava was proven to exhibit antioxidant and cytotoxic activity against MCF-7 breast cancer cell line. These ability were suggested to be related to its alkaloid content: berberine, palmatine, and jatrorrhizine (Keawpradub et al., 2005). Although this plant is stated as a rarely found species (Koran Jakarta, 2012), we could find it abudantly in Meru Betiri National Park, Jember. Our previous studies showed that A. flava leaves increase the immune system in doxorubicin-treated rats (Puspitasari et al., 2014 b ) with no signs of toxicity based on sub chronic toxicity assay (Puspitasari et al., 2014 a ). The A. flava leaves ethanolic extract (EEAfL) had been proven to have cytotoxic activity on HeLa, MCF-7, and WiDr cancer cell lines with the IC 50 value of 467 + 70; 136 + 17; and 213 + 79 µg/ml, respectively. The activity was selective on MCF-7 and WiDr, but not likely on HeLa cell line (Puspitasari et al., 2015).