VOLUNTARY EXERCISE UNDER A FOOD RESTRICTION CONDITION DECREASES BLOOD BRANCHED-CHAIN AMINO ACID LEVELS, IN ADDITION TO IMPROVEMENT OF GLUCOSE AND LIPID METABOLISM, IN DB MICE, ANIMAL MODEL OF TYPE 2 DIABETES

Abstract

Objectuives : Exercise is effective for preventing the onset and development of type 2 diabetes mellitus (T2DM) in human cases; however, the effect of exercise on the pathophysiology using animal models of T2DM has not been fully evaluated, Methods : We applied voluntary exercise under pair-fed (P) conditions in db mice, an animal model of T2DM. Exercising (Ex) and sedntary (Se) mice were placed in a cage, wquipped with a free or locked running wheel, for 4 weeks, respectively. The amount of food consumed by ad libitum-fed wild-type mice under the Se condition (ad-WT) was supplied to all mice, except ad libitum db mice (ad-db). Blood parameters and expression of the genes involved in nutrient metabolism were analyzed. Results: PEx-db (pair-fed and exercising) mice showed significantly lower HbA1c, body weight and liver weight than PSe-db and ad-db mice.