Individual Human Sera Response against Protein Extracts from Salivary Gland of Aedes aegypti

Abstract

The saliva of hematophagous arthropods contains a complex mixture of biologically active proteins. These proteins may modify hemostatic responses and induce both cellular immunity and the production of specific antibodies, thus, influence transmission of its pathogens from arthropods vector to human host. Ae. aegypti is the main vector for transmission of Dengue viruses into human. The objective of this research is therefore to elaborate individual human sera response against protein extracts from salivary gland of Ae. aegypti that mediate the infection of Dengue Viruses. We crossed react human sera from healthy people in endemic and non-endemic area, and dengue patients againts SGE of Ae. aegypti to distinguish to identify the immunogenic proteins by Western Blot Analysis. About 15 protein bands of SGE from Ae. aegypti ranging from 15 kDa up to 255 kDa were identified after 12% SDS-PAGE. 7 dominant bands were detected i.e ~255, 56, 42, 31, 27, 26 and 15 kDa. Two immunogenic proteins (bands) i.e. ~31 and 56 kDa were appeared only in samples from humans who were previously exposed to mosquitoes bites, and not in humans who had not been exposed. Therefore, these immunogenic salivary proteins may serve as indicators for the immune response in humans against protein from salivary gland Aedes aegypti.