ANTIBACTERIAL ACTIVITY OF ETHANOL LEAF EXTRACT OF PURSLANE (Portulaca oleracea) AGAINST Salmonella typhi AND Shigella dysentriae

Abstract

The research has been conducted to examine antibacterial activity of ethanol leaf extract of Purslane (Portulaca oleracea) against Salmonella typhi and Shigella dysenteriae determined by growth on Mueller-Hinton media (in vitro). The research used a posttest-only control group design which used eight times dilution of ethanol leaf extract of Purslane (1 g/ml; 0,5 g/ml; 0,25 g/ml; and 0,125 g/ml). A solution of 0.5 % CMC-Na was used as a negative control. The positive control for Salmonella typhi Chloramphenicol and Siprofloksasin suspension served as positive controls for Shigella dysenteriae, respectively. The results showed that all concentrations of ethanol leaf extracts of Purslane affected the zone of inhibition against Salmonella typhi and Shigella dysenteriae. The extract with concentration of 1 g/ml showed the greatest zone of inhibition against Salmonella typhi (60.25 cfu/ml) and Shigella dysenteriae (25.125 cfu/ml); concentration of 0.5 g/mL (88.25 cfu/ml and 30.125 cfu/ml), concentration of 0.25 g/ml (146 cfu/ml and 38.25 cfu/ml) and concentration 0.125 g/mL (204.25 cfu/ml and 48.25 cfu/ml).