minimal Inhibition concentration of EXTRACT ETHANOL OF PROPOLIS to STAPHYLOCOCCUS AUREUS AND ESCHErichIA COLI

Abstract

The goal of this study is to know the minimal inhibition concentration of extract ethanol of propolis to Staphylococcus aureus and Escheceria coli growths The experimental laboratories research was used to measure inhibition zone which not grow after contact with extract ethanol of propolis on several concentrations with 1 second. Staphylococcus aureus was contact with extract ethanol of propolis that 5000 µg/ml, 2500 µg/ml, 1250 µg/ml, 625 µg/ml, 312,5 µg/ml, 156,25 µg/ml, and 78,125 µg/ml and Escheceria coli was contact with extract ethanol of propolis that 5000 µg/ml, 2500 µg/ml, 1250 µg/ml, 500 µg/ml, 250 µg/ml, 125 µg/ml, 62,5 µg/ml and 31,25 µg/ml concentration. Each concentration was replied for 8 times. To measure the inhibition effect we measured inhibition zone which not grow after contact with extract ethanol of propolis and compared with the control. The data analyzed with one way Annova with 0.05 significancy. The result of this study shown that minimal inhibition concentration of extract ethanol of propolis to Staphylococcus aureus is 312,5 µg/ml and 62,5 µg/ml for Escheceria coli Conclusion of this study is the minimal inhibition concentration of extract ethanol of propolis to Staphylococcus aureus and Escheceria coli growths is 312,5 µg/ml and 62,5 µg/ml