QUALITATIVE STUDY of ESCHERICIA COLI in MINERAL WATER REFILL CENTER in SUMBERSARI, JEMBER.

Abstract

Peoples need water to support their life, in this case water that suitable to drink. Recently, most of them consume refill mineral water which had no quality control. One of indicators of drinking water’s quality for health was Eschericiae coli. E. coli presence in the water indicates that the water was contaminated by fecal material. To evaluate the presence of E.coli bacteria in the mineral water refill center in Sumbersari Jember, the “Bacteriologic water study” was done. Sampling technique used in this study was total sampling. This study use Qualitative approach with 2 stages: Presumptive Test: 3 3 3 tube method to count the Most Probable Number (MPN) of Coliform bacteria, then Confirmed Test: cultivate the positive result on Presumptive Test to the Eosin Metilen Blue (EMB) Agar. Results of this study ; three water sample (33%) had MPN E. coli bacteria more than 0 / 100 ml, grew well on EMB Agar with saw metallic sheen. Conclusion : Three water sample were contaminated by E.coli.