Ekspresi TNF-α Makrofag di Rongga Mulut Tikus Wistar yang diberi Konsumsi Ekstrak Cair Daun Mimba (Azadirachta Indica)

Abstract

Multidimentinal crisis in Indonesia caused to effects the medicine price may not be reached by the citizen, cause citizen choose the traditional medicine. Neem are one ot the trees traditional medicine known by society and has function as immunomodulatory. In the other side TNF- α macrophage were caused to effect activity innate response. TNF- α expression in machrophage in otal has been caused aqueous extract from neem was not explained yet. This research is to explaned there are TNF- α expression in macrophage in oral Wistar. There are 4 groups, one group which was called as control group (KO) had not been feed aqueous extract from neem leaves, the other group which was under treatment classified into 3 groups. First group was fed with 50 mg/day/kg body weight aqueous extract from neem leaves, until day 21 (KP1), second group was fed with 100 mg/day/kg body weight aqueous extract from neem leaves, until day 21 (KP2), third group was fed with 200 mg/day/kg body weight aqueous extract from neem leaves, untik day 21 (KP3). Each group is studied at the day 2. We terminate the rats to take the tongue’s net of rats, so we can make supply and painting for immunohistochemistry. The study showed that there were different results computed using Anova, HSD test, linier regression. Anova shows the significant different (p<0.01) between each groups. The HSD test shows the significant different (p<0.05) each groups. TNF- α be the stakeholders to effort this problems solve, because they are the stimuli sensore from environment, use as parameter to see the effect from the change of innate immune component, aqueous extract from neem leaves increased the macrophage TNF- α expression in oral wistar rats