EKSPRESI SIKLOOKSIGENASE-2 GINGIVA PADA TIKUS YANG DIINDUKSI PERIODONTITIS SETELAH PEMBERIAN EKSTRAK ETANOLIK KULIT MANGGIS

Abstract

Cyclooxygenase is an enzim that synthesis of prostaglandins (PGs) from arachidonic acid. Cyclooxygenase have been characterized and named COX-1 and COX-2. COX-1 is responsible for constitutive PGs production under physiological condition and maintain normal function. On the other hand, COX-2 expression is inducible by cytokines and endotoxin. Periodontitis is a chronic inflammatory disease caused by anaerobic bacteria especially gram negative bacteria. When periodontitis occurred are followed by increased COX-2 expression. Mangosteen rind contains gamma mangostin which inhibits the synthesis of PGE2 thorugh inhibition COX-2 expression.This researches aimed to study COX-2 expression in experimental-induced periodontitis in rats after mangosteen rind etanolic extract administration. Forty eight male wistar rats were induced periodontitis by putting silk ligature subgingivally around the neck of the anterior lower teeth for 7 days. After the ligation was taken out, the rats were divided into 4 groups, and treated orally with mangosteen rind extract 60 mg/kg BB, 30 mg/kg BB, ibuprofen and saline. The rats were sacrified on the 1st, 3rd, 4th, 7th day after the treatment. Their anterior lower jaws were processed for paraffin embedded tissue, cut serially and stained with immuniohistochemistry. COX-2 expression were observed and counted under the microscope (400x).the data were analysed using kruskall wallis test. Kruskal wallis result showed a significant difference COX-2 expression among group indicating that mangosteen rind etanolic extract affected COX-2 expression. In conclusion, mangosteen rind etanolic extract reduced COX-2 expression in periodontitis rats.