Please use this identifier to cite or link to this item: https://repository.unej.ac.id/xmlui/handle/123456789/58332
Full metadata record
DC FieldValueLanguage
dc.contributor.authorNia Kristiningrum-
dc.contributor.authorMia Rakhmawati-
dc.date.accessioned2014-07-14T06:24:12Z-
dc.date.available2014-07-14T06:24:12Z-
dc.date.issued2014-07-14-
dc.identifier.urihttp://repository.unej.ac.id/handle/123456789/58332-
dc.descriptionhttp://www.icpjonline.com/documents/Vol2Issue1/02.pdfen_US
dc.description.abstractA rapid and reproducible TLC method was developed for the determination of hydrocortisone acetate and chloramphenicol in cream. The analytes were dissolved with methanol and chromatographed on silica Gel GF 254 TLC plate using chloroform:ethyl acetate in the ratio of 1:1.5 (v/v) as mobile phase. Spots at Rf 0.29 and Rf 0.59 were recognized as chloramphenicol and hydrocortisone acetate, respectively. Quantitative analysis was done through densitometric measurement at wavelength 265 nm. Method was found linear over the concentration range of 300-900 ng/spot with the correlation coefficient of 0.999 and 0.998 for hydrocortisone acetate and chloramphenicol, respectively. Specificity showed calculation of purity and identity more than 0.99. The limit of detection (LOD) and the limit of quantification (LOQ) of the method were 23.84 and 71.51 ng/spot for hydrocortisone acetate, 21.06 and 63.18 ng/spot for chloramphenicol. The precision of this method was less than 2.8% whereas the means of the recovery data were 100.40± 0.579% for hydrocortisone acetate and 100.24±1.20% for chloramphenicol. The proposed method has been applied to the determination of hydrocortisone acetate and chloramphenicol in commercial cream formulations and the recovery of label claim were 99.23±0.66% (chloramphenicol) and 99.25±0.41% (hydrocortisone acetate) for brand A and 100.32±0.87% (chloramphenicol) and 100.53±0.78% (hydrocortisone acetate) for brand B. The developed method was successfully used for the assay of hydrocortisone acetate and chloramphenicol. The method is simple, sensitive and precise; it can be used for the routine quality control testing of marketed formulations.en_US
dc.language.isootheren_US
dc.relation.ispartofseriesInternational Current Pharmaceutical Journal;2012, 2(1)-
dc.subjectValidation, chromatography, analysis, linearity, reproducible, accuracyen_US
dc.titleSimultaneous determination of chloramphenicol and hydrocortisone acetate in cream using TLC desitrometry methoden_US
dc.typeArticleen_US
Appears in Collections:Fakultas Farmasi

Files in This Item:
File Description SizeFormat 
Nia Kristiningrum_1.pdf361.66 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.