In Vivo Antimalarial Activity of Terpenoid-Rich Fraction of Ethanolic Extract of Tithonia diversifolia (Hemsley) A. Gray Leaves

Abstract

Tithonia diversifolia (Hemsley) A. Gray has been used traditionally to treat malaria. The results of previous study showed that ethanolic extract of this leaves had antimalarial activity in vivo with ED of 113.39 mg/kg. This study aims to fractionated of crude ethanolic extract of T. diversifolia leaves to obtaine terpenoide-rich fraction and to assay it’s antimalarial activity in mice that infected with Plasmodium berghei. Fractionation was conducted by vacuum liquid chromatography using silica gel 60 as stationary phase, and n-hexane, chloroform, methanol as mobile phase. The Antimalarial activity assay carried out using 4day

suppression test. Antimalarial activity assay results indicate that the terpenoide-rich fraction could inhibit the growth of P. berghei ED50 of 5.058 mg/kgBW .