Please use this identifier to cite or link to this item: https://repository.unej.ac.id/xmlui/handle/123456789/58306
Title: A novel high throughput method based on the DPPH dry reagent array for determination of antioxidant activity
Authors: Khalid Hamid Musa
Aminah Abdullah
Bambang Kuswandi
M. Amrun Hidayat
Keywords: Antioxidant activity, DPPH free radicals, 96 well microplate, Dry reagent array
Issue Date: 14-Jul-2014
Series/Report no.: FOOD CHEMISTRY;Volume 141 Issue 4 December 2013
Abstract: A stable chromogenic radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) is commonly used for the determination of antioxidant activity. In this paper, DPPH was dried into 96 well microplate to produce DPPH dry reagent array plate, based on which the highly sensitive and high throughput determination of antioxidant activities was achieved. The spectrophotometric characterization of the microplate containing dried or fresh DPPH free radicals was reported. The response of the DPPH dry reagent array towards different standard antioxidants was studied. The reaction for DPPH in fresh or dry reagent array with Trolox was reported and compared. The DPPH dry reagent array was used to study the antioxidant activity of banana, green tea, pink guava, and honeydew and the results were compared to the samples reacted with freshly prepared DPPH. The proposed method is comparable to the classical DPPH method, more convenient, simple to operate with minimal solvent required and excellent sensitivity.
URI: http://repository.unej.ac.id/handle/123456789/58306
ISSN: 0308-8146
Appears in Collections:Fakultas Farmasi

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