PERANAN DAN MEKANISME KERJA EUGENOL DALAM MENGENDALIKAN NEMATODA SISTA KENTANG (Globodera rostochiensis)

Abstract

The research have been conducted to know the influence and mechanism of eugenol in controlling potato cyst nematode / PCN (Globodera rostochiensis), which is a major parasitic nematodes on potato. Research conducted using complete random design, each treatment repeated six times. Data obtained to be analyzed with the ANOVA and continued with the BNT 5%. Observations were done on percentage of mortality of juvenile phase 2 (J2), hatching eggs in the cyst, the migration ability of J2, Achetycholinesterase inhibition (AChE) J2, non-specific esterase and ATP cyst. The results showed that after exposure to eugenol for 8 hours, J2 was capable of migration as much as 1.33 ± 0.58 while the control tail as much as 19.67 ± 2.51 tail. J2 mortality increased with increasing concentration of eugenol. Concentration of 1 mL / mL has caused J2 mortality above 50% (68.33 ± 5.77%) and J2 mortality reached 100% at concentrations of 10 mL / mL. In observation of hatching eggs in a cyst G. rostochiensis seen that the concentration of 1 mL / mL inhibited hatching eggs permanently. Eugenol inhibits the activity of AChE, non-specific esterase and cyst formation of ATP