The Role of Local Soy-Milk-Honey on Prevention Mandibular bone damage in Rats

Abstract

The soy Milk and The honey respectively have been used to prevent bone damage. Generally, more individual not prefer to consume soy-milk from local soybeans. MLSMH considerated to prevent mandibular bone damage, because it has many substance those have synergistic functions and many biological activity for mandibular bone. The aims of this research are analyses the effects of the local soy-milk from local/Baluran variety, Glysine max (L) Merril mixed by honey from coffe areal estate bees in mixed local soy-milk-honey/MLSMH to proliferation of osteoblast and osteoclast. The methods : 24 male Wistar rats werw wqually divided in 4 groups, such are the saline as control group (group 1), β-APN group (group 2), MLSMH + salin group (group 3), and MLSMH +β-APN (group 4). Experimental alveolar bone damage was inducted by beta-aminopropionitrile (β-APN) once daily subcutaneous administration of at a dose of 5 mg/0,4 ml per 100 g of body weigth for 40 days and intake MLSMH by gaster sondage twice a day at 8-9 a.m and 3-4 P.m. The results under this experiment conditions showed that (1) number of osteoblast decreased in group 2, and increased in group 3 and 4. There are not significant different in group 3 and 4; (2) number of osteoclast very increased in group 2, and decrease in group 3 and 4. There are not significant different between group 1, 3 and 4 (pM>0,05). The conclusion of this research is MLSMH may prevent mandibular bone damage in rats by increasing of osteblast and decreasing of osteoclast.