The number of Macrophages cells of Wistar Rats Gingival after Probiotik Administration Induced by P. gingivalis and LPS E.coli

Abstract

Probiotics are microbes of lactic acid bacteria group that works to maintain health of the host. Probiotic bacteria can stimulate the immune system, among others, increase the function of macrophage phagocytosis, natural killer cells, monocytes and neutrophils, and is able to stimulate the secretion of IgM and IgA production with the end result increasing the production of antibodies locally and systemic. This study aims to determine the effect of probiotics against the macrophage cell number in gingival wistar rats induced P.gingivalis and LPS E.coli. Methods: This study using a sample of male wistar rats and divided into five groups. Group I: is the control without treatment; Group II: induced P.gingivalis ATCC 33277; Group III: induced LPS E.coli; Group IV: induced P.gingivalis ATCC 33 277 + an injection of probiotic (Lactobacillus casei ATCC 4224); group V: induced LPS E. coli + an injection Probiotics (Lactobacillus casei ATCC 4224). The treatment carried out for 5 days, then decapitation were done to take the gingival tissue, after that the histological observation were done to see the number of macrophage cells. Furthermore, the data were analyzed by Kruskal Wallis test, was continued by Mann Whitney test. Results: The result show that there\'s increased of average macrophage cells to all groups; statistically showed the significant difference (p<0,05. Conclusion: Administration of probiotics can increase the number of macrophage cells in wistar rats gingival after induced by P.gingivalis LPS E.coli.