PENGEMBANGAN BIOSENSOR BERBASIS ASETILKOLINESTERASE DENGAN METODE TRANSDUKSI VOLTAMETRI UNTUK SKRINING AKTIVITAS KANDIDAT ANTI-ALZHEIMER PADA GENUS CURCUMA (Curcuma longa Linn. DAN Curcuma xanthorrhiza Roxb.)

Abstract

Alzheimer's disease is physically characterized by involvement of acetylcholine neurotransmitter resulting in disproportionate deficiency of acetylcholine. It has been well known that markers for cholinergic neurons namely acetylcholinetransferase and acetylcholinesterase are responsible for acetylcholine synthesis and its degradation are decreased in the cortex and hyppocampus areas of the brain involved in cognition and memory. An electrochemical biosensor based on acetylcholinesterase immobilized onto screen printed carbon electrode (SPCE) for the investigation of anti-Alzheimer drugs screening was developed. The voltammetric biosensor works based on inhibition of the acetylcholinesterase that has been immobilized onto working electrode surface of SPCE using adsorption method. The degree of inhibition of the acetylcholinesterase enzyme (AChE) by herbal compounds was determined by measuring electrooxidation current of the choline generated by the AChE catalyzed hydrolysis of acetylcholinechloride (AChCl). The biosensor has been applied to determine the IC 50 values of the herbal (rhizome of Curcuma longa Linn. and Curcuma xanthorrhiza Roxb.) as antiAlzheimer’s

candidates. A simultaneous measurement with the differential pulse voltammetry (DPV) and cyclic voltammetry (CV) methods show the applicability of the biosensor for herbal drugs screening.