Activity of Gamavuton-O as Anti Rheumatoid Arthritis on Female Wistar Rat Induced by Complete Freund's Adjuvant with Parameter Suppression of Cytokine IL-1β Level in Joints Tissue

Abstract

Rheumatoid arthritis (RA) is an inflammation dissease in joints that it’s cause still unclearly known. In the some recently study has proven that there is elevation of sitokines level including TNF-α and IL-1β especially in RA joints synovium. Many kind of medicines is used for RA therapy, but thereare still no satisfy result yet and had advers effects to the gastric especially non-steroidal anti-inflammatory drugs (NSAID) that alot used for RA therapy. This study was undertaken to know in vivo activity of Gamavuton-0 (GVT-0) in RA with joints level of IL-1β as parameter. The research has done by inducing female Wistar rats with Complete Freund’s Adjuvant (CFA) to get arthritic rats. After arthritis inducing, the rats perorally administrated with GVT-0 with 10, 20, 40 and 80 mg/kg BW for 21 days and then joints tissue of the rats analyzed by Enzyme Linked-Immuno-Sorbent Assay (ELISA) method to establish IL-1β. The joints level of IL-1β with GVT-0 treatment is compared with normal rats, negative controls (perorally administration with 0,5% Na-CMC ), and positive controls (perorally administration with Metotrexate dose 0,223 mg/kg BB) The research resulting that 80 mg/kg BW of GVT-0 decrease IL-1β in joints tissue by 79,65%. GVT-0 have anti rheumatoid arthritis activity that described as % decreasing of IL-1β (%PKI) with ED50 by 12,38 mg/kg BW.