CULTURE INFLUENCE ON GENETIC VARIATION OF TENGGER

Abstract

Background : Tengger ethnic group that lives in Bromo Highland has a unique history, culture and religion. They culture made them to be married among member of their ethnic (endogamy). Endogamy mating role model could increase the frequency of homozygote genotype. Objective : To explore the genetic variation on Tengger ethnic which their married were influence by culture Material and Methods : 35 blood sample of Tengger people were analyzed. Sample was placed on FTA card. DNA was extracted using FTA purification reagent, and PCR amplification using TPOX, D7S820, D8S1179, FGA and D21S11 primer, subsequently STR typing using PAGE and silver staining method. Results : The results showed 1 dominant allele for TPOX and D8S1179 loci and 2 dominant alleles for D7S820 and D21S11 loci. FGA locus showed almost equal allele frequency of 3 alleles. From 5 STR loci described high level of homozygote genotype on each loci. It happened because the culture influence of their marital aspect. Conclusion : culture could influence in people living environment and could trigger the genetic variation of the population