Minimal Inhibition Concentration of Extract Ethanol of Binahong (Anredera Cordifolia (Ten.) Steenis Leaves to Staphylococcus Aureus

Abstract

The goal of this study is to know the minimal inhibition concentration of extract ethanol of Binahong leaves to Staphylococcus aureus growths The experimental laboratories research was used to measure inhibition zone which does not grow after contact with extract ethanol of Binahong leaves on several concentrations with 1 second. Staphylococcus aureus was contact with extract ethanol of Binahong leaves that 1,95 mg/ml, 3,91 mg/ml, 7,81 mg/ml, 15,62 mg/ml, 31,25 mg/ml, 62,50 mg/ml, 125 mg/ml, and 250 mg/ml concentration. Each concentration was replied for 4 times. To measure the inhibition effect, we measured inhibition zone which does not grow after contact with extract ethanol of Binahong leaves and compared with the control. The data analyzed with one-way Annova with 0.05 significancy. The result of this study shown that minimal inhibition concentration of extract ethanol of Binahong leaves to Staphylococcus aureus is 3,91 mg/ml for qualitative method and 1,387 mg/ml.for quantitative method. Conclusion of this study is the minimal inhibition concentration of extract ethanol of Binahong leaves to Staphylococcus aureus were 3,91 mg/ml for qualitative method and 1,387 mg/ml.for quantitative method.