Identiication of Pili Protein and Outer Membrane Protein (Omp) Of Local Isolate of Shigella Dysenteriae

Abstract

Shigella dysenteriaae is an obligate pathogen that usually found in clinical specimen from shigelosis patients. The pathogenic mechanism of the bacteria is not fully elucidated especially its potential activity of the pili and outer membrane protein (OMP) as hemaglutinin and adhesion molecule. The aim of this study is to predict the molecule weight of pili and OMP from S. dysenteriae. After identification, bacterial isolate of pili and outer membrane protein fraction 12,5 % SDS-PAGE were used to isolate OMP followed by hemaglutinin test and invitro adhesion test. The study showed that the molecule weight protein of S. dysenteriae were 155 kDa, 124 kDa, 78 kDa, 32 kDa and 117 kDa, 84 kDa, 73 kDa, 52 kDa,35 kDa for OMP.