Ovarian Failure Affected Leukocytes Profile in Peripheral Blood and Gingival Fluid (In vivo Study)

Abstract

Ovarian failure can be caused by ovariectomy which induces estrogen deficiency. Estrogen influenced leukocytes as immune and inflammation cells systemically and locally, and leukocytes also contribute to ovarian function in order to be located in ovaries tissue. The aim of the study was to investigate the impact of ovarian failure to leukocytes profile in peripheral blood circulation and gingival fluid. This study was experimental laboratory study approved by the Health and Research Ethics Committee of Dental Faculty, Gadjah Mada University. The animal models have undergone ovariectomy which removed their ovarian bilaterally. Blood sampling from retro orbital plexus and gingival fluid were taken before and 3, 7, 14, 21 and 28th days after ovariectomy procedures. Total leukocytes counted manually. The result showed that total leukocytes in peripheral blood were higher than in gingival fluid. Moreover, there were significant differences between leukocytes profile in peripheral circulation and gingival fluid based on the periods, except in basophils cells counting. The conclusion was ovarian failure affected leukocytes profile in peripheral blood circulation and gingival fluid.