In Vitro Analysis of Human IgG Immune Response Against 31 kDa and 67 kDa Immunogenic Protein from Aedes albopictus Salivary Glands

Abstract

Mosquito salivary glands contain protein substances that can facilitate dengue virus transmission. Those proteins have abilities to induce an immune response and specific antibody production. Previous studies showed that 31 kDa and 67 kDa protein fractions from Aedes albopictus salivary glands are immunogenic protein. They can be recognized by the human IgG antibodies of endemic residents from Jember Regency by the Western Blot analysis. However, the IgG levels are not known quantitatively. Therefore, this study is expected to decide the level of IgG as a human humoral immune response against 31 kDa and 67 kDa protein fractions from Ae. Albopictus salivary glands. We separated salivary gland proteins using SDS-PAGE and continued using the electroelution method to get purified protein fraction. These proteins then cross-reacted with human blood serum using Indirect ELISA. The results showed that the IgG level against 31 kDa protein fraction based on the absorbance value in the healthy human population, DHF patients, and neonates were 0.229, 0.198, 0.151 respectively in individual serum and 0.229, 0.198, 0.151 in pool serum. While the IgG level against 67 kDa protein fraction based on the absorbance values in the individual serum of healthy human population, DHF patients, and neonates were 0.4293, 0.3159, 0.2997 respectively and 0.410, 0.096, 0.037 in pool serum. Those results indicate that the IgG antibodies of each population group can well recognize the 31 kDa and 67 kDa protein fractions. The highest levels of IgG found in healthy residents, followed by DHF patients, and neonates.