Humoral Immune Response (IgG) of BALB/c Mice (Mus musculus) Post-injection by 56 kDa Immunogenic Protein Extract from the Salivary Glands of Aedes aegypti L.

Abstract

The primary mosquito vector of dengue virus isAe. aegypti, and dengue virus transmitted by saliva mosquito’s during blood feeding. The saliva composed of numerous proteins with capacity to disrupt hemostasis or modulate the host immune response. The previous study identified two immunogenic proteins in the salivary glands of Ae. aegypti i.e. 56 kDa and 31 kDa. However, the mechanism of host’s humoral immune response (IgG) against the 56 kDa immunogenic protein is not known. Therefore, this study was conducted to observe the mice strain BALB C immune response (IgG) against the 56 kDa. The mice were divided into three groups of injection treatments, particularly Group-A (Tris-Cl 0.05M), Group-B (adjuvant), and Group-C (56 kDa immunogenic protein 0.1 μg/μL + adjuvant). Injection was done every two weeks in total six weeks of treatment. The IgG level was measured every two weeks using ELISA method. The study results exhibited the Group-C had higher IgG level than Group-A and Group-B. The IgG level of Group-C gradually increased along the increasing of 56 kDa protein exposures, meanwhile the IgG level of Group-A and Group-B tended to be steady before and after treatment. These results suggested that repeated exposure of 56 kDa immunogenic protein elevated the humoral immune response (IgG) in host.