Mechanism of Purple Leaves Extract in Reduce the Number of Lymphocytes in Traumatic Ulcer

Abstract

Background: Purple leaves are one of the medicinal plants that have various benefits, one of which is as an anti-inflammatory. Flavonoids in purple leaves work as an anti-inflammatory, one of which is in conditions of traumatic ulcers which in the process show symptoms of inflammation such as redness and pain. In chronic inflammation that occurs in traumatic ulcers, inflammatory cells, one of which are lymphocytes, will migrate to the trauma area and cause symptoms of inflammation. Inflammation as much as possible is limited because if it continues it can cause the wound to undergo an abnormal healing process so that it continues to become pathological inflammation. This study aims to determine the effect of giving purple leaves extract on the number of lymphocytes in traumatic ulcers of wistar rats. Method: There were five treatment groups namely normal group, control group, treatment group one (extract 0.05 g/kg BW), treatment group two (extract 0.15 gr/kg BW), and treatment group three (extract 0,45 g/kg BW). Rats were euthanized on the 7th day for histopathological preparations. The data in this study were tested using the One-way Anova parametric statistical test. Result: All treatment groups significantly reduce the number of lymphocytes. The highest decrease occurred in the group with the highest dose, presumably because the higher the dose, the more active substances were able to reduce the number of lymphocytes. Conclusion: At a dose of 0.05 g/kg BW rats could significantly reduce the number of lymphocytes in tissues that had traumatic ulcers.