In vitro studies on Bacillus sp. and Pseudomonas sp. compatibility with botanical pesticide

Abstract

The effect of botanical pesticides as an insect pest control agent has been proven in several studies. Despite capable of controlling pests, botanical pesticides have not been reported to promote plant growth. One technique for making botanical pesticides served in dual function is to combine them with beneficial microbes. This study was aimed to determine the compatibility of 3 isolates of Bacillus sp. and 3 isolates of Pseudomonas sp. against botanical pesticides. The botanical pesticides were made from a mixture of Ageratum conyzoides, Aglaia odorata, and Azadirachta indica leaves. The solvent used 96% ethanol, and the process of making botanical pesticides was performed using a rotary evaporator at 45˚C. The compatibility test was performed in vitro by dropping 10 µl of botanical pesticides on the bacterial culture. Tests were carried out using various concentrations, namely 0%, 25%, 50%, 75%, and 100%. The test results showed that Bacillus sp. A and Pseudomonas sp. E strain were compatible on all assay concentrations. Bacillus sp. A and Pseudomonas sp. D and Pseudomonas sp. E were compatible with the concentrations of 0 to 75%. Furthermore, Bacillus sp. B, Bacillus sp. C, and Pseudomonas sp. F were compatible with the assay concentrations of 0 to 50%.