Virulence of Steinernema spp. an entomopathogenic nematodes Indonesia isolates against larvae of white grub Lepidiota stigma F (Coleoptera: Scarabaediae) in the laboratory condition

Abstract

Lepidiota stigma (F) (Coleoptera; Scarabaeidae) is the key pest in sugarcane plantation caused devastated damaged on roots. The objectives of this research are to determine virulence of Steinernema sp, an entomopathogenic nematodes to larvae of L. stigma in laboratory. The Experiment was conducted in the laboratory, Agrotechnolofy Department University of Jember. Filter paper and sand method was used to determined the effective concentration of entomopathogenic nematodes againts larva of white grub.The result showed that all entomopathogenic nematodes able to kill larvae of white grub L. stigma at all level concentration and soil depth. The result also convinced that Steinernema sp. origin from Kediri was more virulence compared with the other isolates.