Extracellular matrix types and distribution of pattern differentiation of ameloblastoma based on histopathological type appearance

Abstract

Background. The stromal tumor as Extracellular Matrix (ECM) of Ameloblastoma is a complex structures surrounding intercellular and extracellular that supporting cells motility and regulation of cell proliferation, cells shape, and their function in connective tissue. Histological appearance of Ameloblastoma ECM particularly are fibrocollagen and fibromyxoid. The activation of stromal tumor predicted to increase tumor aggressiveness by stimulation of angiogenesis and invasiveness. Several author reported that there were several pattern of stromal tumor in the body such as focal, network and spindle pattern. Purpose. The purpose of this study to identify and analyze the ameloblastic stromal tumor pattern and their distribution based on their histological appearance type. Methods. Using fifteen paraffin embedded tissue taken from Jember district hospital that were surgically treatment from year 2010 to 2014 and diagnosed as ameloblastoma. All specimen cutted 4 µm and then performed HE and Mallory trichrome staining. All cases observed and analyzed using light microscope with cubical micrometer scale (graticulae) 400x magnification. Results. ECM pattern of Ameloblastoma cases were 5 cases plexiform type showed network pattern, 1 case was focal pattern and another case was spindle. Whereas 4 cases of follicular type showed spindle pattern, 2 cases were network pattern and 2 cases were focal. Conclusion. The stromal tumor of ameloblastoma types particularly network pattern (54%) that distributed between tumor nest, this pattern perhaps due to local invasiveness and aggressiveness.