Please use this identifier to cite or link to this item: https://repository.unej.ac.id/xmlui/handle/123456789/106326
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dc.contributor.authorWASILAH, Ummi-
dc.contributor.authorMUZAKHAR, Kahar-
dc.contributor.authorPURWATININGSIH, Purwatiningsih-
dc.date.accessioned2022-04-11T06:55:27Z-
dc.date.available2022-04-11T06:55:27Z-
dc.date.issued2020-07-25-
dc.identifier.govdocKODEPRODI1810401#Biologi-
dc.identifier.govdocNIDN 0003056808-
dc.identifier.urihttp://repository.unej.ac.id/xmlui/handle/123456789/106326-
dc.description.abstractIn this study, microbial utilization of coffee pulp waste to produce three carbohydrases (cellulase, pectinase, and xylanase) has been investigated. An isolate Listeria sp. ISH-16 which grown in liquid medium containing 0.5% coffee pulp alkali extract can grow well with density of reach 107 x 58.5 cell/mL. Further, the culture can optimum produced of pectinase after 72 hours incubation at 37oC, but for cellulase and xylanase longer 84 hours incubation needed. The harvested of crude pectinase, cellulase and xylanase had activity 0.15, 0.23, and 0.61 unit/ ml after dialysis, respectively. All enzymes stable at a range of pH 4-5.5, but they work optimum at different pH. Of each enzyme pectinase, cellulase, and xylanase work optimum at pH 5, 4.5, and 5.5. based on these results, it may be adopted as a strategy to produce enzymes with low cost so that further investigation such as large scale and purification enzyme is needed.en_US
dc.language.isoenen_US
dc.publisherIPTEK Journal of Proceedings Seriesen_US
dc.subjectCarbohydraseen_US
dc.subjectCellulaseen_US
dc.subjectCoffee Pulpen_US
dc.subjectPectinaseen_US
dc.subjectXylanaseen_US
dc.titleCellulase, Pectinase, and Xylanase Production by Listeria sp. ISH 16 using Coffee Pulp Waste Mediumen_US
dc.typeArticleen_US
Appears in Collections:LSP-Conference Proceeding

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