Decomposition of Coffe Pulp Polysaccharides By Aspergillus Niger Extraselluler Enzyme

Abstract

Coffee pulp is agricultural waste which amount of annual production capacity of 336 tons in Indonesia at 2012. The coffee pulp containing a major component in the form of polysaccharides consisting of cellulose, hemicellulose, lignin and C / N ratio is relatively high which causes this agricultural waste takes longer to decompose naturally. Aspergillus niger strains B10 MCC-00136 is a fungus that can produce a wide variety of extracellular enzymes that can be utilized for the decomposition process. A. niger strain B10 MCC-00136 was grown on medium coffee pulp and crude enzyme to be extracted then carried out using a reducing sugars assay method Somogy Nelson. The optimum activity was obtained on day 5th with reduction sugars produced by 29.9 ug / ml. Testing results crude production of enzymes at pH 3.5 to 6 and a temperature of 30-50 °C showed optimum activity at pH 4, 5 and a temperature of 40 oC. The results of crude enzyme hydrolysis test reaches optimum at the 18th hour with an efficiency of 4,69 %.