Extraction Bioactive Compound of Pegagan (Centella Asiatica L.) using Solvent-Free Microwave-Assisted Extraction

Abstract

Gotu Kola (Centella asiatica L.), known as pegagan is a plant with many benefits, including used to repair burns, hypertrophic wound healing, and as an inflammatory agent. C. asiatica grows in tropical Asia and grows in various countries such as Indonesia, Philippines, China, India, Sri Lanka, and Madagascar. C. asiatica has four bioactive components, namely asiatic acid, asiaticoside, madecassic acid, and madecassoside. Microwave-Assisted Extraction (MAE) is one of the methods for extracting bioactive compounds of pegagan leaf. In this study, the extraction of pegagan bioactive compounds carried out using the Solvent-Free MicrowaveAssisted Extraction (SFME) method. This research used a box-behnken design model for the optimization process. The variables used are mass of material (20, 40, 60 g), power (150, 300, 450 W), and time of extraction (30, 60, 90 min). The highest yield is 4,5474% at 450 watt microwave power, 20 g mass pegagan leaves, and 60 min extraction time. Mass of material is the factor that has significant effect on yield. Modelling from this research is appropriate which has lack-of-fit > 0,1, and R2 89,67%. The content of bioactive compounds carried out by analyzing the total phenolic content of the product. The most substantial phenol content is 2398,649 μgAGE/ml.