Isolation protocol of jumbo phage from winter grass soil

Abstract

Jumbo phage can isolated by two different methods and protocols. It depends on size, phage species, substrate sources, environment, media and tools. Each method has positive and negative results within each factors. The research aimed to determine the suitable protocol to isolate the jumbo phages from winter grass soil during winter conditions, utilizing two main protocols, the enrichment protocol and non-enrichment protocol. In Sequent, research was conducted with several steps, including bacterial isolation, jumbo phage isolation, plaque test in Escherichia coli colony, bacterial genus identification, phage test in soil bacteria, protocol evaluation, DNA extraction and electrophoresis. Based on the plaque size and plaque total, each protocol did not significantly differ on plaque total among the E. coli or each isolated soil bacteria. On the E. coli test, the plaque total average was 65 plaque, nor 45 plaque emerge on each isolated soil bacteria. The significant difference on each protocol was on the clarity of plaque. Based on visual observation, the plaques with enrichment protocol were clearer. It was obvious that the isolation of jumbo phage from the winter grass during winter conditions can be done without enrichment protocol. It can be isolated with standard isolation protocol