Expression of TLR-2 of Mice Infected by Mycobacterium Tuberculosis by Administration of Methanol Extract of Graftophyllum pictum L. Griff

Abstract

Tuberculosis is a chronic infectious disease caused by Mycobacterium tuberculosis. Toll-Like Receptor-2 (TLR-2) signaling plays a role in the body's defense system against Mycobacterium tuberculosis. Daun Ungu (Graptophyllum pictum L. Griff) is a traditional herbal plants which has medicinal properties for infectious and inflammatory diseases. This study aimed at analyzing the expression ofTLR- 2 of mice with the administration of methanol extract of Daun Ungu (Graptophyllum pictum L. Griff). The method used was an experimental laboratory, thirty male mice aged 8-12 weeks were randomly allocated into 5 groups; 2 control groups (KO, K 1 ), 3 treatment groups (PI, P2, P3 ). KI, PI, P2 and P3 were infected using Mycobacterium tuberculosis at a dose of 108 ml tuberculosis. Macrophages are professional phagocytic cells with main function to destroy inununogen and as and left for 4 weeks. At the fifth week groups PI, P2 and P3 were administered with methanol extract of Graptophyllum pictum L. Griff (EMDU) at a dose I, 703 mg, 3 .406 mg, 6. 812 mg. The administration of 0.2 ml was conducted every other day for 2 weeks. Examination of the expression of TLR-2 was carried out using immunohistochemical techniques. The data were analyzed using ANOV A and correlation analysis. The results showed that the EMDU in mice infected with Mycobacteriwn tuberculosis by imununohistochemical technique was succeed that the EMDU could increase the expression of TLR-2. The results of the correlation test showed a positive correlation between dose EMDU with TLR-2 expression. The conclusion of this study is that the EMDU can increase expression ofTLR-2.